Enzyme immunoassay of vitamin d3 metabolites
نویسندگان
چکیده
منابع مشابه
Enzyme immunoassay for measuring 25-hydroxyvitamin D3 in serum.
We developed a rapid, competitive enzyme immunoassay (EIA) for measuring 25-hydroxyvitamin D3 [25(OH)D3] in serum. The EIA was based upon 25(OH)D3-3-hemisuccinate covalently coupled to secondary amino groups grafted onto the polystyrene surface of microtiter wells. Optimal coupling conditions were established, and we found that inclusion of 40 mumol/L chloramine T, an agent not previously descr...
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FIG. 1 Separation of the specific protein binding with vitamin D3 and its metabolites in rat intes tinal cytoplasm by gel filtration on Sephadex G 200 after the administration of vitamin D3-3H in vivo. 1,000I.U. (3•~105cpm) of randomly labeled vitamin D3 (sp. act. 1.2•~104cpm/ug) was injected intracar dially to vitamin D deficient rat 16 hours before decapitation. 2.0 ml of rat intestinal cytop...
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PURPOSE This study was designed to measure vitamin D metabolites in the aqueous and vitreous humor and in tear fluid, and to determine if dietary vitamin D3 supplementation affects these levels. We also determined if the corneal epithelium can synthesize vitamin D following UV-B exposure. METHODS Rabbits were fed a control or vitamin D3 supplemented diet. Pilocarpine-stimulated tear fluid was...
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Two new vitamin D metabolites were isolated in pure form from incubations of 53 nM 1,25-dihydroxyvitamin D3 with homogenates of small intestinal mucosa of vitamin D-replete chicks. The birds were injected intravenously with 8 to 9 nmol of 1,25-dihydroxyvitamin D3/100 g body weight 5 to 8 h before death. The isolation involved methanol-chloroform extraction and four successive chromatographic pr...
متن کاملEffects of vitamin D3 and calcium supplementation on serum levels of tocopherols, retinol, and specific vitamin D metabolites.
γ-Tocopherol (γT) protects against DNA-damaging effects of nitrogen oxides, yet its physiologic regulation in vivo is unknown. Observational studies indicate inverse associations of 25[OH]-vitamin D with γT and leptin. To determine whether vitamin D(3) supplementation alters levels of lipid-soluble micronutrients, serum samples (N = 85 subjects) from a randomized, double-blind, placebo-controll...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences of Belarus, Chemical Series
سال: 2020
ISSN: 2524-2342,1561-8331
DOI: 10.29235/1561-8331-2020-56-2-197-205